Figure 4.

In C2C12 cells, ROCK1 activates PTEN, which impairs Akt activity. (A) PTEN activity assay indicates that caspase-3 activation stimulates PTEN activity (mean±SEM, n=3). (B) Akt activity was measured by the level of phosphorylated GSK3β from cells with or without caspase-3 activation. (C) Increased PTEN activity stimulated by caspase-3 is blunted by the ROCK1 inhibitor (fasudil) (mean±SEM, n=3). (D) Western blot reveals Akt activity is higher after ROCK1 inhibitor was added despite activation of caspase-3.