Figure 1.

Benefit of BR-4628 (10 mg/kg) administration against renal injury induced by IR. Renal function was assessed by quantification of the plasma levels of (A) creatinine and (B) urea. As indicators of tubular injury: (C) the urinary levels of heat shock protein 72 were determined by western blot and the bands scanned for densitometric analysis and the mRNA levels in the kidney of kidney injury molecule-1 (D) and neutrophil gelatinase–associated lipocalin (E) were determined by RT-PCR. (F) The percentage of injured tubules was blindly quantified in ten fields per rat. Representative images from the hematoxylin-and-eosin staining are shown for sham (G), IR (H), BR-4628 pre (I), and BR-4628 post (J). Scale bar, 100 μm. n=5 per group. *P<0.05 versus sham and Φ P<0.05 versus IR.