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. 2016 Jan 28;11(1):e0147212. doi: 10.1371/journal.pone.0147212

Table 1. Primer of rat gene used for quantitative realtime PCR analysis.

Gene name Primers sequences
TNFα For:5′-gctcacaatgtctgtgcttagag-3′; Rev:5′-gcagtagccacagctccag-3′
IL-1β For:5′-tgtgatgaaagacggcacac-3′; Rev:5′-cttcttctttgggtattgtttgg-3′
NLRP3 For:5′-cagcgatcaacaggcgagac-3′;Rev:5′-agagatatcccagcaaacctatcca-3′
VEGF For: 5′-ctacctccaccatgccaagt-3′; Rev:5′-gcagtagctgcgctgataga-3′.
CD31 For:5′-cttcaccatccagaaggaagagac-3′; Rev:5′-cactggtattccatgtctctggtg-3′
Angiopoetin-1 For: 5′-gggaggttggactgtaat-3′; Rev:5′-ctttatcccattcagttt-3′.
iNOS For: 5′-agcatcacccctgtgttccaccc-3′; Rev:5′-tggggcagtctccattgcca-3′.
eNOS For: 5′-ctgctgcccgagatatcttc-3′; Rev:5′-cag gta ctg cag tcc ctcct-3′
18S For:5′-gtaacccgttgaaccccatt-3′; Rev:5′-ccatccaatcggtagtagcg-3