Figure 7. Centrosome separation is defective in Nup88^T and Nup98^+/− Rae1^+/− MEFs.

(A) Images of Nup88^T MEFs immunostained for γ-tubulin and p-HH3 (Ser10). (B) Quantification of the incidence of G₂-phase cells of the indicated genotypes with unseparated centrosomes treated with or without BI2536. (C) Same as in B. (D) Schematic representation of spindle geometric measurements and representative images of metaphases immunostained for α-tubulin and γ-tubulin with the indicated spindle angle. (E) Quantification of spindle geometric abnormalities for MEFs of the indicated genotypes. (F) Same as in E. DNA in A and D was visualized with Hoechst. Quantifications in B, C, E, and F were performed on 3 independent lines per genotype (20 cells/line). Data represent the mean ± SEM. Statistical significance in B and C was determined using a 1-way ANOVA, followed by Tukey’s multiple comparisons test. Statistical significance in E and F was determined using a 2-tailed, unpaired t test. *P < 0.05 and **P < 0.01. Scale bars: 10 μm. Nup88^T indicates the combined transgenic lines 11 and 13.