Skip to main content
. 2015 Dec 21;126(2):560–570. doi: 10.1172/JCI79197

Figure 2. SNARK protects against palmitate-induced apoptosis in myocytes.

Figure 2

C2C12 myoblasts were treated with scrambled or SNARK siRNA to generate SNARK-knockdown cells. (A) Snark mRNA (relative to B2m housekeeping gene) (control: n = 5 wells, siRNA: n = 6 wells) and (B) protein levels were measured, with AMPK shown as a loading control. Average protein levels were quantified from n = 6 wells per condition. Following 24 hours of transfection with siRNA, scrambled (CON) or SNARK siRNA (siRNA) cells were treated with vehicle (VEH; ethanol in 2% BSA) or 250 μM palmitate for 24 hours. (C) Viable cells in each treatment group were quantified using the MTT proliferation assay. The average results from 3 independent experiments (n = 6 wells per condition per experiment) are shown. (D) Western blotting was performed on cell lysates to analyze MYPT1/Rho kinase and apoptotic signaling. AMPK is shown as a loading control. The average protein levels were quantified from 3 wells per group and are representative of 3 independent experiments. C-Casp 3, cleaved caspase-3. *P < 0.05 vs. CON; #P < 0.05 vs. VEH of same treatment group determined by t tests (A and B) and 2-way ANOVA with Bonferroni post-hoc testing (C and D). Error bars indicate mean ± SEM.