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. 2016 Jan 5;126(2):605–610. doi: 10.1172/JCI84428

Figure 1. Broadly binding and neutralizing anti-HA stalk mAbs require Fc-FcγR interactions for protection in vivo.

Figure 1

(A) Broadly neutralizing anti-stalk (red lines), strain-specific anti-head (blue lines), broadly neutralizing anti-head (green lines), and non-neutralizing anti-head (purple lines) mAb binding to Neth09-infected cells by flow cytometric analysis and (B) neutralization of Neth09 virus (mean ± SEM) in duplicate samples. (C and D) WT mice were given the indicated doses of IgG2a (red circles) or DA265-mutant (blue squares) FI6 mAb (C), 2G02 mAb (D), or PBS (black triangles) before Neth09 virus infection. Values represent the mean ± SEM percentage of weight change compared with day-0 values (left panels) and percentage of survival (right panels). n = 4–5 mice per group. Significant differences between the IgG2a and DA265 samples are shown. *P < 0.05 and **P < 0.01, by 2-tailed Student’s t test.