Figure 3. Myofibroblast transition represses EPO.

(A) Hematocrit of UUO mice with or without phlebotomy 1 day before analyses at the indicated time points. n = 10 per group per time point. (B) Expression of Epo and Phd3 in CL and UUO kidneys. n = 10 per group per time point. (C) Expression of Epo, Vegfa, and Phd3 of Col1a1-GFP⁺PDGFRβ⁺ pericytes and myofibroblasts isolated from CL kidneys and kidneys 7 days after UUO surgery from Col1a1-GFP^Tg mice, respectively. n = 4 per cell group. (D) Epo expression of pericytes and myofibroblasts cultured in the presence of IOX2 for 24 hours. n = 4 per group. (E) Plasma levels of BUN and creatinine in mice fed with regular chow or chow containing 0.25% adenine for 21 days. n = 10 per group. (F–H) Hematocrit, renal Epo expression, and plasma EPO levels of mice fed with regular or adenine chows. n = 10 per group. (I) Epo expression of Col1a1-GFP⁺PDGFRβ⁺ pericytes and myofibroblasts isolated from kidneys of Col1a1-GFP^Tg mice fed with regular and adenine chows, respectively. n = 4 per cell group. One-way ANOVA was used for analyses of data in A–C and E–H, and Student’s t test was used for analyses of data in D. *P < 0.05, ^†P < 0.01, ^‡P < 0.001.