Fig 3. In vivo Analysis of Mutant Integrated trm6-504 Strains.
A) Phenotypes of chromosomal integrated copies of wild-type or single mtr4 mutants expressed in trm6-504 strains by serial dilution spot assays on YPD at 30°C or 36°C. B) Northern blot of total RNA to detect steady-state tRNAiMet levels in trm6-504 bearing wild-type or mutant MTR4. 5S rRNA was detected as a loading control. The percentage of mature tRNAiMet from each strain was calculated after normalization to 5s rRNA and is expressed relative to trm6-504/MTR4-WT C) Total protein western blot from trm6-504/MTR4 and trm6-504/mtr-x detecting Mtr4p and Nab2p, a loading control.