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. 2016 Jan 28;11(1):e0148090. doi: 10.1371/journal.pone.0148090

Fig 4. In vivo Analysis of Mtr4p-K904N Mutant Expressing on High Copy Plasmid in trm6-504.

Fig 4

A) Growth of trm6-504/MTR4 transformant bearing high-copy plasmid-born (HC) wild-type MTR4 or HC mtr4-904, by a serial dilution spot assay and incubation on SC-Ura at 30°C or 36°C. Growth of WT yeast strain TRM6/MTR4 was used as a positive control. B) Northern blot of total RNA to detect tRNAiMet steady-state levels in trm6-504 strains bearing HC MTR4-WT, or HCmtr4-904. 5S rRNA was detected and used to normalize the amount of tRNAiMet in each lane, and the percentage of tRNAiMet from each strain is shown relative to trm6-504. C) Western blot analysis of total protein to detect Mtr4p in trm6-504 strain with HC MTR4, or HC mtr4-904, and Nab2p is detected as a loading control.