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. 2016 Jan 28;11(1):e0148090. doi: 10.1371/journal.pone.0148090

Table 1. Used Plasmids.

Plasmid Description
B184 YEplac195 vector
B187 YCplac111 vector
B451 His-tagged MTR4 ORF in pET15b expression vector
B534 Flag-tagged TRF4 and His-Tagged AIR2 co-expressed on Petduet vector
B568 A 4kb fragment containing entire MTR4 gene cloned into YEplac195 high copy number plasmid
B573 mtr4-K904N mutant created from B451 by site-directed mutagenesis (Stratagene)
B576 mtr4-K700N mutant created from B451 by site-directed mutagenesis (Stratagene)
B577 mtr4-P731S mutant created from B451 by site-directed mutagenesis (Stratagene)
B579 mtr4-R1030G mutant created from B451 by site-directed mutagenesis (Stratagene)
B587 mtr4-K904N mutant on Yeplac195 vector rescued from trm6-504 in dominant-negative screen