Table 4. Structural Domain Mutant Library by Random Mutagenesis.
| Structural Domain Mutants | Mutations |
|---|---|
| Mutant 3–1* | T712P |
| Mutant 4–1* | P731S |
| Mutant 4–2* | S672N |
| Mutant 4–3* | L903M |
| Mtr4-22 | R883C |
| Mtr4-23 | K700N, R1030G |
| Mtr4-24 | F673L, R678K, F924S, Q946H |
| Mtr4-25 | P802S, M825I, A965T |
| Mtr4-26 | M825V, Y853F |
| Mtr4-27 | N925D |
| Mtr4-28 | F698Y, P802S, A965T |
| Mtr4-29 | F945S, F1010Y |
| Mtr4-30 | K904N |
| Mtr4-31 | V1019A |
| Mtr4-32 | D899N |
| Mtr4-33 | K768M |
*indicate mutants identified during development of error-prone PCR for this application, all the others were identified by the dominant-negative screen (Materials and Methods). Mutations in the arch domain are shown with Italic font. Mutations in the winged-helix domain are indicated by bold, and the remaining mutations are in the ratchet domain.