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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Cell Metab. 2015 Sep 10;22(4):606–618. doi: 10.1016/j.cmet.2015.08.018

Figure 5. Exosomes secreted due to MSCT downregulated intracellular levels of miR-29b in recipient MRL/lpr BMMSCs.

Figure 5

(A) Western blot showed Dnmt1, Notch1 and NICD expression in BMMSCs. (B) Alizarin red staining showed mineralized nodule formation by BMMSCs. n = 4. (C) Real-time PCR showed miR-29b expression in BMMSCs. n = 5. (D) Western blot showed Dnmt1, Notch1 and NICD expression in BMMSCs. (E) Alizarin red staining showed mineralized nodule formation of BMMSCs. n = 5. (F) Western blot showed Runx2 and ALP expression in BMMSCs. (G) Real-time PCR showed miR-29b expression in BMMSCs. n = 5. (H) Western blot showed Dnmt1, Notch1 and NICD expression in BMMSCs. (I) Alizarin red staining showed mineralized nodule formation by BMMSCs. n = 5. Western blot showed Runx2 and ALP expression in BMMSCs. (J) H&E staining showed formation of new bone (B) and bone marrow (BM) around HA/TCP (HA) carrier when BMMSCs were implanted into immunocompromised mice. n = 5. (K) μCT analysis of BMD and BV/TV of femurs. n = 5. All results are representative of data generated in three independent experiments. Statistical significance was determined with one-way ANOVA. **P < 0.01. Error bars: mean ± SD, 200 μm (J), 1 mm (K).