A1, Brn3AP positive areas include central stations of the vagus nerve (AP, NTS, and dmnX), in an adult Phox2b:Cre; Brn3bCKOAP animal. A2 B, AP signal in the anatomic position of the NA (black arrow head) in Phox2b:Cre; Brn3bCKOAP (A2) and cRetCreERt; Brn3bCKOAP (B) mouse. C, D Immunostaining analysis did not reveal any Brn3b expression in the Chat positive dmnX (Figure 12C stippled circle) or hypoglossal nuclei (12C circle). Expression was limited to the Area postrema (arrow), subdividions of the nucleus of the solitary tract (C arrow head) and NA (D). Scale bars: A1,A2,B=500μm, C,D=50μm.