Figure 3. Dysregulated lysosomal activity in microglia of rraga^st77/st77 mutants.

(A–B) Analysis of RNA sequencing data revealed that genes associated with lysosomal activity are significantly upregulated genes in rraga^st77/st77 mutants at 5 dpf. Using the DAVID Bioinformatics Resources, genes showing at least a 1.5-fold upregulation were classified accord (A) Kegg Pathway terms and (B) molecular functions (GOTERM_MF_FAT). (C–D) Visualization of Lysotracker Red and Tg(mpeg1:EGFP) in wildtype (C, n = 4) and rraga^st77/st77 mutants (D, n = 4) reveals that lysosomes are increased in microglia in the mutants. Larvae shown in C and D were genotyped by PCR after photography.