Fig. 10.

c-Fos interacts with HDAC3 and Htt. a HEK293T cells were transfected with EGFP-c-Fos along with pLKO.1, HDAC3-Flag, or JAZ-Flag. After 24 h, lysates were collected and were subjected to immunoprecipitation using either IgG or Flag antibody. Samples were then subjected to Western blot analysis. b HEK293T cells were transfected with Flag-c-Fos. Twenty-four hours later, lysates were collected and subjected to immunoprecipitation using either IgG or Flag antibody. Samples were then analyzed using Western blotting, and the presence of HDAC3 was detected. c HEK293T cells were transfected with HDAC3-Flag and EGFP-c-Fos (360–380). Twenty-four hours later, lysates were collected and subjected to immunoprecipitation using IgG, Flag, or GFP antibody. Samples were then analyzed using Western blot. d HEK293T cells were transfected with Flag-c-Fos along with pLKO.1, RelA-GFP, or Q15-Htt-GFP. After 24 h, lysates were collected and were subjected to immunoprecipitation using either IgG or Flag antibody. Samples were then subjected to Western blot analysis. e CGN cultures were treated with HK medium for 6 h. Lysates were collected, and immunoprecipitation was performed using IgG and Htt antibody. Samples were then subjected to Western blot analysis