Fig. 9.

c-Fos rescues CGNs from HDAC3-induced apoptosis. CGN cultures were transfected with HDAC3-Flag along with pLKO.1, EGFP-c-Fos (a), or EGFP-c-Fos (360–380) (b). Eight hours after transfection, cells were treated with either HK or LK medium. Immunocytochemistry was performed 24 h after treatment, and cell viability of transfected cells was assessed and normalized to an EGFP-transfected control. Results were obtained from at least three separate experiments done in duplicates. Statistical analysis using Student’s t test was performed where *** represents p<0.0001