Skip to main content
. Author manuscript; available in PMC: 2017 Jan 28.
Published in final edited form as: Genesis. 2015 Aug 10;53(9):612–626. doi: 10.1002/dvg.22879

Figure 3. Evc2 homozygous mutant mice show dwarfism.

Figure 3

(a–b) LacZ staining showing Evc2 expression pattern of growth plates of indicated bones from wild type (a) and heterozygous Evc2 (b) mice. (c) Dorsal view of an Evc2ex12/ex12 mutant mouse with littermate controls at the newborn stage. (d, e) Dorsal view of Evc2ex12/ex12 mice with littermate controls at 1 week (d) and 6 weeks (e) after birth. (f) Side view of skeleton preparation of Evc2 ex12/ex12 and a littermate control at 4 weeks after birth. (g) Body weight gains of male and female Evc2 ex12/ex12 (red) and littermate controls (blue) were shown respectively (*, p<0.05, **, p<0.01). (h) Skeletal preparation of limbs from E18.5 Evc2 ex12/ex12 and littermate control indicated shorter forelimb and hindlimb in the Evc2 mutant. (i) Quantification of the length of indicated appendicular bone in (h) (n=10, *, p<10−10). Hu, humerus; Ul, ulna; Ra, radius; Fe, femur; Ti, tibia. (j) Skeleton preparation of limbs from E18.5 Evc2dE/dE embryos and littermate controls indicated shorter forelimb and hindlimb in the Evc2 mutant. (k) Quantification of the length of indicated appendicular bone in (j) (n=10, *, p<10−10). Scale Bar: 200 um for a and b, 1 mm for h and j.