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. 2016 Jan 27;11(1):e0146783. doi: 10.1371/journal.pone.0146783

Fig 1. Prophylactic treatment with rHA-Infestin-4 reduces infarct area and brain edema formation after tMCAO in rats.

Fig 1

(A) Upper panel shows representative 2,3,5-triphenyltetrazolium chloride stainings of 3 coronal rat brain sections 24 h after transient middle cerebral artery occlusion (tMCAO) in rats. Rats were treated with 100 mg/kg rHA-Infestin-4 or saline (control) 15 min prior to tMCAO. tMCAO was held up for 90 min followed by reperfusion until 24 h. Corresponding individual infarct areas, analyzed by planimetry, are shown in the lower panel. Prophylactic rHA-Infestin-4 treatment significantly reduced infarct areas compared to saline treated rats (n = 29-31/group). **p<0.01, Mann-Whitney test compared to saline treated animals. Dots above the dashed horizontal line indicate dead animals per group. Mortality was included in statistical analysis. Horizontal solid lines indicate mean values (+SEM) of surviving animals. (B) In addition, brain edema formation 24 h after tMCAO was reduced in rHA-Infestin-4 treated rats compared to saline treated control rats (n = 29-31/group). **p<0.01, Mann-Whitney test compared to saline treated animals. Dots above the dashed horizontal line indicate dead animals per group. Mortality was included in statistical analysis. Horizontal solid lines indicate mean values (+SEM) of surviving animals.