Table 2.
NMR restraints used for the structure determination.
| XPC | p62 | |
|---|---|---|
| Distance restraintsa | ||
| Intramolecular NOEs | ||
| 13C-edited NOESY-HSQC (τm, 100 ms) | 73b | 1367c |
| Intraresidue (i–j=0) | 7b | 171c |
| Sequential (i–j=1) | 48b | 204c |
| Medium-range (1<i–j<5) | 18b | 233c |
| Long-range (i–j≥5) | 0b | 759c |
| 15N-edited NOESY-HSQC (τm, 150 ms) | 109d | 1178e |
| Intraresidue (i–j=0) | 10d | 197e |
| Sequential (i–j=1) | 86d | 419e |
| Medium-range (1<i–j<5) | 13d | 223 e |
| Long-range (i–j≥5) | 0d | 339e |
| Intermolecular NOEs | 216 | 156 |
| 13C,15N-filtered 13C-edited NOESY-HSQC (τm, 120 ms) | 162 | 107 |
| 13C,15N-filtered 15N-edited NOESY-HSQC (τm, 150 ms) | 54 | 49 |
| 13C,15N-filtered 13C-edited NOESY-HSQC (τm, 120 ms) | 199f | |
| 13C,15N-filtered 15N-edited NOESY-HSQC (τm, 150 ms) | 100g | |
| Dihedral restraints | ||
| Φ | 9h | 96i |
| Ψ | 9h | 95i |
| χ1 | 3h | 63i |
| χ2 | 0h | 10i |
| Hydrogen bond restraints | ||
| 0 | 96 (48×2)j | |
a
Distance restraints were obtained from analyses of NOE intensities by using NMRView [3].
b
Supplementary Table S1.
c
Supplementary Table S3.
d
Supplementary Table S2.
e
Supplementary Table S4.
f
Supplementary Table S5.
g
Supplementary Table S6.
h
Supplementary Table S7.
i
Supplementary Table S8.
j
Supplementary Table S9.