More specific subject area |
Psychoneuroendocrinology and molecular biology |
Type of data |
Figures |
How data was acquired |
Real-time PCR (QuantStudioTM 6 Flex Real-Time PCR System, Applied Biosystems, Foster City, CA) |
Data format |
Analyzed |
Experimental factors |
Male Wistar rats were subjected to acute restraint stress for 0 (control), 60, 120, and 240 min in the dark-cycle. At the end of each treatment, whole brain was rapidly removed and the medial prefrontal cortex was cryo-dissected for relative gene expression analysis. |
Experimental features |
Total RNA was extracted from each isolated medial prefrontal cortex, reversed transcribed to cDNA, and the relative expression of neuronal nitric oxide synthase (NOS), inducible NOS, and I kappa B alpha (Nfkbia) was determined. |
Data source location |
Brisbane, Australia |
Data accessibility |
Data is within this article |