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. 2016 Jan 28;11(1):e0147868. doi: 10.1371/journal.pone.0147868

Fig 4. Effect of different MWs of HA on the MSC-mediated induction of Tregs.

Fig 4

Frequency of Tregs (CD25+CD127-) within CD4+ T cells is shown (A, B). A) Effect of MSC donor-to-donor variability and different MWs of HA on Treg frequency. B) Effect of IFNγ-supplemented conditions on the Treg ratio from CD4+ T cells when MSCs and/or HAs are present. (A) and (B) show data from two separate experiments; normal conditions are shown in (B) for comparison purposes with IFNγ- supplemented conditions. Solid filled bars stand for hHA; hatched bars, 150 kDa HA; dotted bars, 7.5 kDa HA and empty bars, no HA. Black color, normal conditions and gray color with red frame, IFNγ-supplemented conditions. Each bar indicates the mean of triplicates replicates from MSCs or Th cell from one donor. Error bars represent 95% CI. * p<0.05, ** p<0.01 and ***<0.001.