Table 1. Effect of HAs on the expression of selected MSC transcripts (fold change relative to no HA control).
| Transcript | 1.6 MDa HA | 150 kDa HA | 7.5kDa HA | no HA | 1.6 MDa HA+PBMC-CM | 150 kDa HA+PBMC-CM | 7.5 kDa HA+PBMC-CM | no HA+PBMC-CM |
|---|---|---|---|---|---|---|---|---|
| CD44 | 1.2 ±0.2 | 1.1 ±0.2 | 1.0 ±0.2 | 1.0 ±0.1 | 0.9 ±0.1 | 0.9 ±0.1 | 0.8 ±0.1 | 0.8 ±0.1 |
| IL6 | 1.0 ±0.1 | 1.0 ±0.1 | 1.0 ±0.3 | 1.0 ±0.3 | 2.1 ±0.2†† | 2.3 ±0.2††† | 2.3 ±0.3†† | 1.6 ±0.9 |
| COX2 | 0.7 ±0.1 | 0.6 ±0.1 | 0.6 ±0.2 | 1.0 ±0.4 | 0.7 ±0.1 | 0.9 ±0.1 | 0.7 ±0.1 | 0.8 ±0.1 |
| SOX9 | 0.9 ±0.2 | 0.8 ±0.1 | 0.8 ±0.1 | 1.0 ±0.3 | 0.6 ±0.1 | 0.6 ±0.1 | 0.6 ±0.1 | 0.7 ±0.1 |
| PRG4 | 0.9 ±0.2 | 0.9 ±0.1 | 0.8 ±0.3 | 1.0 ±0.3 | 0.6 ±0.1 | 0.7 ±0.1 | 0.6 ±0.2 | 0.7 ±0.3 |
| TLR4 | 1.1 ±0.0 | 0.9 ±0.1 | 1.0 ±0.3 | 1.0 ±0.1 | 0.7 ±0.5 | 1.0 ±0.0 | 0.9 ±0.1 | 0.9 ±0.1 |
| ICAM1 | 1.2 ±0.2 | 1.1 ±0.2 | 1.0 ±0.3 | 1.0 ±0.1 | 18.0 ±2.3††† | 17.2 ±2.6††† | 17.9 ±2.0††† | 17.8 ±3.0††† |
| CYCLIND1 | 1.4 ±0.1 | 1.1 ±0.2 | 1.2 ±0.3 | 1.0 ±0.4 | 1.1 ±0.2 | 0.9 ±0.1 | 0.9 ±0.1 | 0.9 ±0.1 |
| NFKBIA | 1.3 ±0.4 | 1.3 ±0.5 | 1.2 ±0.3 | 1.0 ±0.1 | 1.5 ±0.2 | 1.4 ±0.2 | 1.4 ±0.3 | 1.4 ±0.2 |
| CXCL8 | 0.4 ±0.1 | 0.5 ±0.0 | 0.5 ±0.2 | 1.0 ±0.7 | 5.0 ±0.5††† | 5.1 ±1.0††† | 5.1 ±0.7††† | 4.9 ±1.0††† |
| MMP3 | 1.0 ±0.4 | 0.8 ±0.2 | 0.9 ±0.2 | 1.0 ±0.1 | 1.1 ±0.4 | 1.0 ±0.1 | 1.0 ±0.2 | 0.6 ±0.6 |
| ACAN | 1.1 ±0.1 | 1.3 ±0.6 | 1.0 ±0.1 | 1.0 ±0.1 | 0.8 ±0.2 | 0.8 ±0.1 | 0.8 ±0.1 | 0.8 ±0.1 |
| VEGFA | 0.8 ±0.1 | 0.8 ±0.1 | 0.8 ±0.1 | 1.0 ±0.2 | 0.6 ±0.1 | 0.5 ±0.1†† | 0.6 ±0.0† | 0.7 ±0.1††† |
| PDL1 | 0.7 ±0.4 | 0.7 ±0.3 | 0.7 ±0.4 | 1.0 ±0.7 | 1.4 ±0.2 | 1.5 ±0.5 | 1.5 ±0.5 | 1.6 ±0.6 |
| PDL2 | 1.1 ±0.2 | 1.2 ±0.5 | 1.1 ±0.2 | 1.0 ±0.2 | 1.5 ±0.3 | 1.4 ±0.1 | 1.4 ±0.2 | 1.3 ±0.1 |
| HGF | 1.2 ±0.3 | 0.9 ±0.1 | 1.0 ±0.2 | 1.0 ±0.2 | 1.0 ±0.1 | 0.9 ±0.2 | 0.9 ±0.2 | 0.9 ±0.2 |
| TGF-B | 1.2 ±0.2 | 1.0 ±0.1 | 1.0 ±0.1 | 1.0 ±0.1 | 0.8 ±0.0†† | 0.8 ±0.1 | 0.7 ±0.1†† | 0.9 ±0.2 |
| TSG-6 | 1.0 ±0.1 | 1.0 ±0.1 | 1.1 ±0.2 | 1.0 ±0.2 | 0.7 ±0.0†† | 0.7 ±0.0† | 0.8 ±0.1†† | 0.8 ±0.0 |
| IDO a | - | - | - | - | +††† | +††† | +††† | +††† |
Expression is shown as mean± 95% CI of three experimental replicates from a representative MSC sample after 4 hours of exposure to HA. Relative transcript expression was also normalized to the expression of B2M. Highlights:
†<0.05;
††<0.01 and
††† <0.001 relative to similar counterpart without PBMC-conditioned medium (CM).
a Shown as + and - expression, no effects from HA when expressed.