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. 2016 Jan 29;7:9. doi: 10.3389/fphys.2016.00009

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Copyright © 2016 Baker Bechmann, Rotoli, Morales, Maeso, García, Ávila, Mobasheri and Martín-Vasallo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of the Na,K-ATPase β1 and β2 isoforms in normal liver tissue and liver metastasis. (A) β1 isoform is mainly located at the cytoplasmic membrane of hepatocytes (arrows) from normal liver. (B) Bile duct epithelial cells (B) express β1, V, venule. (C) Nuclei of metastatic tumor cells (arrows). (D) β1 IHC-positive staining at necrotic tissue is signaled with arrows; S, septum. (E) β2 is differentially expressed at the cytoplasm of neighboring hepatocytes (arrows) of normal liver. (F) Hepatocytes with different degrees of β2 immunostaining at a peri-nuclear position in neighboring hepatocytes (arrows) of normal liver. (G) In normal liver, a bile duct (B) and cells from the lining connective tissue (arrow) express β2. (H) Metastatic tumor cells (T) do not express β2.