Table 1.
Antibodies used in this study. α1(620) (Sztul et al., 1987), α3 (Pietrini et al., 1992), α3 (XVIF9-G10) (Arystarkhova and Sweadner, 1996), SpETβ1 and SpETβ2 (Gonzalez-Martinez et al., 1994).
| Antibody | Target | Host | Type | Dilution | Source |
|---|---|---|---|---|---|
| α1(620) | α1-isoform* | R | P | 1:1000 | M. J. Kashgarian |
| α3 | α3-isoform* | R | P | 1:600 | M. Caplan |
| α3 (XVIF9-G10) | α3-isoform* | M | Mc | 1:5 | Arystarkhova and Sweadner |
| SpETβ1 | β1-isoform* | R | P | 1:600 | P. Martin-Vasallo |
| SpETβ2 | β2-isoform* | R | P | 1:600 | P. Martin-Vasallo |
| Anti-proliferating cell antigen (Anti-PCNA) | PCNA | M | Mc | 1:100 | Boehringer Mannheim |
| Anti-rabbit IgG (H+L), biotin conjugated (2°) | Rabbit-IgG | G | P | 1:300 | Pierce |
| Anti-rabbit IgG (whole molecule), FITC-conjugated (2°) | Rabbit-IgG | G | P | 1:200 | Sigma |
| Anti-mouse IgG, DyLight®650-conjugated (2°) | Mouse-IgG | G | P | 1:100 | Abcam |
R, rabbit; G, goat; M, mouse; Mc, monoclonal; P, polyclonal.
*
subunit-isoforms of the Na,K-ATPase, 2°: secondary antibody.