Table 2.

Cell-specific Na,K-ATPase subunit-isoform expression in normal colon, colorectal cancer, normal liver, and metastasized liver.

	α1	α3	β1	β2
Normal colon
Epithelial cells (Mucosae)	+++	+++	+++	+
Mesenchymal cells (Submucosae)	+	+	+	+
Smooth muscle cells (Muscularis mucosae)	?	?	?	?
Neurons (Myenteric plexus)	?	+	+++	?
Glia cells (Myenteric plexus)	?	?	+++	+++
Smooth muscle cells (Muscularis propia)	?	+	−	+
Colorectal cancer
Tumor cells	+++	+++	+++	−
Mesenchymal cells (not immune system cells)	+	+++	−	+
Immune system cells	?	+++	?	?
Endothelial cells	?	+++	−	?
Epithelial cells (Mucosae)	+++*	+++	+++	−
Normal liver
Hepatocytes	+++	−	+++	+
Ephitelial cells (bile duct)	+++	−	+	+
Endothelial cells	−	−	−	−
Mesenchymal cells (connective tissue)	−	−	−	+
Metastasized liver
Tumor cells	+++	+++	+	−
Hepatocytes	?	+	?	−*
Epithelial cells (Bile duct)	+++	?	?	?
Mesenchymal cells (connective tissue)	−	+	−	−
Immune system cells	−	+++	?	?

+++, indicates a high staining level; +, indicates low staining level; −, indicates no staining detected; and ?, indicates indeterminate staining, according to our observations.

^*Data not shown.