Figure 3.

Dynamics of early and late endosomes in root hairs. (A) Kymographs, a line (width = 1px) along a cytoplasmic strand in the middle of a cell (subapical), show movements of the early endosomes visualized by GFP-RabA1d and YFP-VTI12 and late endosomes visualized by YFP-RabF2a, YFP-RabF2b, and GFP-2xFYVE. Single slice image acquisition was performed (50 ms exposure time and 10–15 frames per second). (B) The maximum speed of endosomes was determined in kymographs by eye (steepest slopes) in mature root hairs. More than 39 endosomes from over 20 cells of at least 10 individual plants were analyzed for each endosomal compartment. (C) Proportional ratio between continuous and discontinuous movements of endosomes. Non-interrupted movements along a straight line, at least 15–20 μm long, were assigned as continuous while all other types of movements were assigned as discontinuous ones. This classification was performed on the same kymographs used in (B). Different letters above the bars indicate significant difference from Wilcoxon rank sum test with Holm's correction (p < 0.05).