FIGURE 6.

Co-expression of GalNAc-T2 and C1GalT1 results in the modification of the hinge region peptide. (A) Co-expression of GalNAc-T2 leads to the incorporation of HexNAc residues. (B) Co-expression of GalNAc-T2 and C1GalT1 results in the generation of core 1 structures: HexNAc + hexose (Hex). (C) Incubation with jacalin agarose (+) reveals binding of the sIgA1 alpha chain when modified in planta with mucin-type O-glycan biosynthesis enzymes. The amount of sIgA1 before binding to jacalin is also shown (-). Human serum IgA was used as a positive control and sIgA1 alone or co-expressed with the N-glycan modifying β1,4-galactosyltransferase (GalT) was used as negative control.