Table 1. The results of 2HG detection by 1H–MRS.
Tn, true-negative; Tp, true-positive; Fn, false-negative; Fp, false-positive; U, uncertain case (CRLB >20%); n/a, not available;
| MRUI | LCModel | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rat number (tissue type) | FID Truncation | Voxel-specific baseline | Simulated baseline | Voxel-specific baseline | ||||||||||
| 2HG /water | 2HG CRLB(%) | Detectability | 2HG /water | 2HG CRLB(%) | Detectability | Detectability (2HG cut-off* consideration) | 2HG /water | 2HG CRLB(%) | Detectability | 2HG /water | 2HG CRLB(%) | Detectability | Detectability (2HG cut-off# consideration) | |
| Group A (2HG-absent) | ||||||||||||||
| #1 (F98 IDH1-WT) | 0.00 | n/a | Tn | 0.00 | n/a | Tn | Tn | 2.83 | 20 | Fp | 1.69 | 47 | U | Fp |
| #2 (F98 IDH1-WT) | 0.00 | n/a | Tn | 0.67 | 31 | U | Fp | 3.32 | 15 | Fp | 0.28 | 217 | U | Tn |
| #3 (F98 IDH1-WT) | 0.00 | n/a | Tn | 0.03 | 424 | U | Tn | 2.89 | 21 | U | 0.77 | 104 | U | Fp |
| #4 (F98 IDH2-WT) | 0.00 | n/a | Tn | 0.27 | 54 | U | Fp | 2.22 | 18 | Fp | 0.00 | 999 | Tn | Tn |
| #5 (F98 IDH2-WT) | 1.37 | 21 | U | 0.00 | n/a | Tn | Tn | 3.28 | 13 | Fp | 1.46 | 36 | U | Fp |
| #6 (F98 IDH2-WT) | 0.70 | 85 | U | 0.00 | n/a | Tn | Tn | 4.50 | 17 | Fp | 0.00 | 999 | Tn | Tn |
| #7 (CN VOI) | 0.00 | n/a | Tn | 0.00 | n/a | Tn | Tn | 2.82 | 20 | Fp | 0.76 | 74 | U | Fp |
| #8 (CN VOI) | 0.00 | n/a | Tn | 0.00 | n/a | Tn | Tn | 2.69 | 20 | Fp | 0.28 | 217 | U | Tn |
| #9 (CN VOI) | 0.00 | n/a | Tn | 0.00 | n/a | Tn | Tn | 3.28 | 18 | Fp | 0.86 | 83 | U | Fp |
| #10 (CN VOI) | 0.00 | n/a | Tn | 0.00 | n/a | Tn | Tn | 2.72 | 23 | U | 0.00 | 999 | Tn | Tn |
| #11 (CN VOI) | 0.00 | n/a | Tn | 0.00 | n/a | Tn | Tn | 2.04 | 24 | U | 0.00 | 999 | Tn | Tn |
| #12 (CN VOI) | 0.00 | n/a | Tn | 0.00 | n/a | Tn | Tn | 3.05 | 21 | U | 0.80 | 100 | U | Fp |
| Group B (2HG-present) | ||||||||||||||
| #13 (F98 IDH2-R172K) | 0.03 | 447 | U | 5.52 | 7 | Tp | Tp | 4.12 | 8 | Tp | 5.31 | 15 | Tp | Tp |
| #14 (F98 IDH2-R172K) | 2.30 | 10 | Tp | 2.91 | 7 | Tp | Tp | 6.80 | 11 | Tp | 4.42 | 24 | U | Tp |
| #15 (F98 IDH2-R172K) | 4.09 | 6 | Tp | 2.37 | 9 | Tp | Tp | 3.83 | 9 | Tp | 3.71 | 13 | Tp | Tp |
| #16 (F98 IDH2-R172K) | 0.00 | n/a | Fn | 0.48 | 54 | U | Tp | 5.75 | 13 | Tp | 6.76 | 19 | Tp | Tp |
| #17 (F98 IDH2-R172K) | 3.97 | 5 | Tp | 8.64 | 5 | Tp | Tp | 7.49 | 10 | Tp | 5.32 | 16 | Tp | Tp |
| #18 (F98 IDH2-R172K) | 0.00 | n/a | Fn | 5.65 | 7 | Tp | Tp | 4.12 | 20 | Tp | 0.00 | 999 | Fn | Fn |
| #19 (F98 IDH2-R172K) | 0.81 | 23 | U | 2.22 | 8 | Tp | Tp | 5.81 | 9 | Tp | 3.29 | 18 | Tp | Tp |
| Correct detection | 13 (68%) | 15 (79%) | 17 (89%) | 7 (37%) | 9 (47%) | 12 (63%) | ||||||||
| Incorrect detection | 2 (11%) | 0 (0%) | 2 (11%) | 8 (42%) | 1 (5%) | 7 (37%) | ||||||||
| Uncertain case (CRLB >20%) | 4 (21%) | 4 (21%) | 0 (0%) | 4 (21%) | 9 (47%) | 0 (0%) | ||||||||
*2HG/water cut-off for 2HG absent voxel = 0.20;
#2HG/water cut-off for 2HG absent voxel = 0.52