Figure 2.

(A) Hemolysis in stored blood on storage Days 5 and 42. Scatter dot plots show the median (interquartile range) values for cell-free plasma hemoglobin (CFPHb), arginase-1 (Arg-1), and lactate dehydrogenase (LDH) for subjects. Values for CFPHb (n = 18), Arg-1 (n = 15), and LDH (n = 15) were statistically significant when comparing levels at Day 5 of storage with Day 42 of storage, using Wilcoxon matched-pairs signed rank testing. (B) Correlation of nitric oxide (NO) consumption with CFPHb in stored blood for 5- and 42-day samples. Samples from the stored blood unit were obtained before transfusion for measure of analytes in the supernatant. NO consumption highly correlated with measured levels of CFPHb on Day 5 (n = 18) and Day 42 (n = 18), both of which are statistically significant.