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. 2015 Nov 15;5(12):3505–3515.

Figure 3.

Figure 3

Knockdown of C4.4A decreases migration and invasion of HNSCC cell line. A. Expression of C4.4A protein in HNSCC cell lines was higher than normal keratinocytes cell line (OKC) as the Weston Blot results showed (Mean ± SD; *, P < 0.05, **, P < 0.01, ***, P < 0.001, One-way ANOVA); B. Knockdown of C4.4A by two different siRNA (Mean ± SD; *, P < 0.05, **, P < 0.01, ***, P < 0.001, Two-way ANOVA); C. Wound healing assay showed knockdown of C4.4A suppressed the cell mobility of CAL27 cell line, and quantification of cell numbers with ImageJ “cell counter” module shows the statistical significance of the difference (Mean ± SD; **, P < 0.01, student t-test with GraphPad Prism5.0); D and E. Transwell assay showed the migration and invasion abilities of CAL27 were impaired after knocking down of C4.4A compared with those of control group, and quantification of cell numbers with Image J “cell counter” module (Mean ± SD; **, P < 0.01, ***, P < 0.001, student t-test with GraphPad Prism5.0).