Figure 1.

Metformin inhibited human salivary gland cell growth in vitro. A-C: HSY cell viability assay. HSY cells were plated and cultured in DMEM medium and received metformin at different concentrations 0 mM, 2 mM, 4 mM, or 8 mM for three different time points. Crystal violet assays were done, the OD values were read via plate reader. (ANOVA were used to determine the significant difference. **P < 0.01, ***P < 0.001). D: Images (20 ×) were captured on 48 hours treated group prior to cell viability assay. E-H: HSG cell viability assay. HSG cells were cultured in MEM medium and treated by metformin as previous described in HSY cells. (ANOVA were used to determine the significant difference. ***P < 0.001).