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Metformin arrested human salivary gland carcinoma cell cycle in S/G2 phases. A, B: HSY cells were treated with PBS or 4 mM metformin for 48 hours. PI was used to stain the cells. **P < 0.01. C: HSY cells were treated for 48 h and the protein was harvested, then the cell cycle markers were tested by Western blot.
