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Metformin but not PP242 reduced c-MYC expression in human salivary gland cells. A: HSY cells were evenly plated in 24 well plates and treated in 6 groups for 48 h: PBS, DMSO: 0.5 µl/mL, Metformin: 4 mM, PP242: 0.5 µM, Metformin+PP242: 4 mM+ 0.5 µM, Metformin+DMSO: 4 mM+ 0.5 µL/mL. B: Images (200 ×) were captured prior to CV assay. C: Total protein was extracted from HSY cells, Phospho-4E-BP1, p-AMPK, p53 and MYC levels were tested by Western blot.
