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Metformin stimulated apoptosis in human salivary gland cells. (A, B) HSY cells were treated with 4 mM metformin for 48 hours. Annexin V and PI were used to stain the cells. **P < 0.01, ***P < 0.001. (C, D) Western blot using both nuclear and total protein with relevant apoptosis markers (C: nuclear protein).
