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. 2015 Nov 15;5(12):3635–3644.

Figure 4.

Figure 4

miR-610 down-regulates HDGF expression by directly targeting its 3’UTR. A. Schematic representation of the construction of wild-type or mutant HDGF 3’UTR vector. B. The luciferase reporter assay was performed in HEK293T cells co-transfected with wild-type or mutant HDGF 3’UTR vector with miR-610 mimic or controls. C and D. HDGF protein and mRNA expression in HCT116 and SW480 cells treated with lentivirus-expressing miR-610 or negative control were measured by western blotting and qRT-PCR, respectively. E. The mRNA expression of HDGF in 33 paired CRC samples and adjacent noncancerous tissues. F. Scatter plots showing the negative correlation between miR-610 and HDGF mRNA expression in CRC tissues. *P < 0.05, **P < 0.01.