Figure 1.

Protective effect of vitamin D in liver IR injury. A. Hepatic function was assessed by measuring serum ALT and AST concentrations. B. Six hours after reperfusion, sham and IR livers pretreated with vehicle and vitamin D were fixed in 10% buffered formalin and processed in paraffin. 4-μm sections were cut and stained with Hematoxylin and eosin. C. The histological degree of hepatic IR injury was assessed according to the Suzuki’s criteria. D. Liver paraffin sections were analyzed for apoptosis using DAPI and TUNEL assay, apoptotic index indicates that the vitamin D treated group had significantly less TUNEL-positive cells when compared to the control group. Data represent mean ± S.D. (*p<0.05, **p<0.01).