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Vitamin D increases hepatocellular autophagic flux in vitro. (A) Hepatocytes were treated with vitamin D in the presence/absence of bafilomycin A1, wortmannin or rapamycin. The protein samples were collected eight hours after the reoxygenation. Relative LC3-II (B) and SQSTM1 (C) level were determined by densitometry and normalized to GAPDH levels. Data represent mean ± S.D. (*p<0.05, **p<0.01).
