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. 2015 Dec 15;7(12):2661–2671.

Figure 6.

Figure 6

miR-135a overexpression protects against hypoxia/reoxygenation (H/R) induced increase of ROS production and apoptosis levels in HL-1 cells exposed to high glucose. A: HL-1 cells with the treatment of H/R were cultured in 30 mM glucose. MiR-135a mimic (or pre-NC) and pcDNA-TXNIP (or pcDNA) were transfected into cells for 24 h. The intracellular ROS level and cell apoptosis were measured by DCFH-DA method and TUNEL assay, respectively. B: HL-1 cells with the treatment of H/R were cultured in 5.5 mM glucose. The intracellular ROS level and cell apoptosis were detected in HL-1 cells transfected with miR-135a mimic (or pre-NC) and pcDNA-TXNIP (or pcDNA). Values are expressed as means ± SEM. Each experiment was repeated three times. **P<0.01 vs. cells exposed to 5 mM or 30 mM glucose. ##P<0.01 vs. cells treated with H/R and pre-NC. &P<0.05 vs. cells treated with H/R, miR-135a mimic and pcDNA.