Figure 1.

Production of EndoC-βH3 cells a Tamoxifen inducible excisable human beta cell line derived from EndoC-βH2. (A) Schematic representation of lentiviral vector used to produce EndoC-βH3 cells expressing both the TAM inducible form of CRE (CRE-ERT2) and the resistance to puromycin (B) Cells were passaged every week. Amplification index was defined as fold change between the initial number of seeded cells and the one counted one week later before cell passage. Data represent average of amplification index ± S.E.M. over 10 passages of EndoC-βH3 cells cultured with or without antibiotic (puromycin) selection.