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. 2015 Oct 20;4(12):916–925. doi: 10.1016/j.molmet.2015.09.008

Figure 2.

Figure 2

Definition of optimal procedure for efficient Tamoxifen treatment of EndoC-βH3. (A) Schematic representation of the experimental optimization procedure for TAM treatment. (B, C, E) EndoC-βH3 cells were treated for 7 days (white box) or 21 days (Gray box) with TAM. Un-treated cells are represented as hatched box. Proliferation assays and insulin content measurements were performed at day 21. (B) Effect of TAM treatment on SV40LT gene expression. Quantitative PCR results are representative of 2 independent experiments performed in triplicates. Data corresponded to average relative expression values normalized to TPB expression ± S.E.M. (C–D) Effect of TAM treatment on EdU incorporation by EndoC-βH3 cells (C) or EndoC-βH2 (D) relative to total cell number. EdU was added for 1 h and analysis was performed by FACS. Results are representative of 2 independent experiments performed in duplicates. Data are expressed as average percentage ± S.E.M. (E) Effect of TAM treatment on insulin content in EndoC-βH3. Data are expressed as insulin content per million cells ± S.E.M. of 3 independent wells per condition assayed in triplicates by ELISA.