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Glucose responsive insulin secretion in EndoC-βH3 following treatment with Tamoxifen. EndoC-βH3 were treated or not for 21 days with 1 μM TAM. (A) Glucose stimulated insulin secretion (GSIS) on un-treated EndoC-βH3 in presence or absence of IBMX. (B) GSIS on TAM-treated EndoC-βH3 in presence or absence of IBMX. (A–B) GSIS data are expressed as ng of secreted insulin per hour ± S.E.M. of 3 independent wells seeded with 7 × 104 cells per condition assayed in triplicates by ELISA.
