Figure 6.

Long-term phenotype of Tamoxifen treated EndoC-βH3. (A) Cell growth monitoring over time (every 7 days) during 63 days. TAM treatment was either stopped at day 21 (in gray), or continued during the whole experiment (in black). (B) Insulin content was determined in EndoC-βH3 cells continuously treated with TAM and compared to untreated cells. Analysis was performed every second week, starting 21 days after initial TAM treatment. Data are expressed as fold change over untreated cells (dotted line) of insulin content ± S.E.M. of 3 independent wells per condition assayed in replicates by ELISA. (C–D) Gene expression by quantitative RT-PCR was determined in EndoC-βH3 cells that were continuously treated with TAM and compared to untreated cells. Analysis was performed every second week, starting 21 days after initial TAM treatment. Data are expressed as fold change of mRNA expression relative to TBP ± S.E.M. of 3 independent RNA preparations assayed in triplicates.