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Effect of single ICV injections of glucagon (120, 240, 480 or 1000 ng/rat) on cumulative food intake (A), measured after 1, 2, 4, 6, 8 and 24 h. Effect of ICV glucagon (480 ng/rat) injections on neuropeptide mRNA expression (B) and protein levels (C) measured after 1 h. β-actin was used to normalize protein levels. Dividing lines indicate spliced bands from the same gel. Values are mean ± SEM of 7–8 animals per group. *P < 0.05; **P < 0.01; ***P < 0.001.
