Figure 2.
Effect of ICV glucagon receptor antagonist (des-His1 (Glu9) glucagon amide) (0.5, 1, and 2 μg/rat) on cumulative food intake, measured after 4 h (A). Food intake in rats injected ICV with vehicle, glucagon, glucagon receptor antagonist, and glucagon with a previous injection of the glucagon receptor antagonist (B). Effect of the injection of lentiviral particles encoding a green fluorescent protein (GFP) or the glucagon receptor in the ARC (C) and ARC protein levels of glucagon receptor (D). Cumulative food intake after overnight fasting (12 h) after the injection of lentiviruses encoding shRNA against glucagon receptor into the ARC of rats fed a chow diet (E). β-actin was used to normalize protein levels. Dividing lines indicate spliced bands from the same gel. Values are mean ± SEM of 7–8 animals per group. *P < 0.05; **P < 0.01.