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. 2015 Oct 22;4(12):961–970. doi: 10.1016/j.molmet.2015.09.014

Figure 3.

Figure 3

Effect of ICV vehicle and the PKA activator Sp-cAMPS (90 ng/rats) injections on cumulative food intake in rats fed a chow diet (A). Food intake (B) and hypothalamic protein levels (C) in rats fed a chow diet injected ICV with vehicle, glucagon, PKA inhibitor (H-89), and glucagon with a previous injection of H-89. β-actin was used to normalize protein levels. Dividing lines indicate spliced band from the same gel. Values are mean ± SEM of 7–8 animals per group. *P < 0.05; **P < 0.01; ***P < 0.001.