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. 2015 Oct 22;4(12):961–970. doi: 10.1016/j.molmet.2015.09.014

Figure 4.

Figure 4

Fluorescein-isothio-cyanate (FITC) staining in the hypothalamic arcuate nucleus (ARC) (A), glucagon protein levels in the ARC (B), food intake (C) and ARC protein levels (D) in rats after injection of glucagon specifically in the ARC. FITC staining in the hypothalamic ventromedial nucleus (VMH) (E), glucagon protein levels in the VMH (F), food intake (G) and VMH protein levels (H) in rats after injection of glucagon specifically in the VMH. Effect of the injection of adenoviral particles encoding for a green fluorescent protein (GFP) or constitutively active AMPK (AMPKα-CA) in the ARC (I). Food intake (J) and ARC protein levels (K) were measured 1 h after ICV injection with glucagon. β-actin was used to normalize protein levels. Dividing lines indicate spliced bands from the same gel. Values are mean ± SEM of 7–8 animals per group. *P < 0.05; **P < 0.01; ***P < 0.001.