Skip to main content
. 2015 Oct 22;4(12):961–970. doi: 10.1016/j.molmet.2015.09.014

Figure 5.

Figure 5

Effect of ICV glucagon (480, 1000 ng/rat) and GLP-1 (10 μg/rat) injections on cumulative food intake in rats fed a high fat diet (HFD) after 2, 4, 6 and 8 h (A). Effect of ICV glucagon (480 ng/rat) injection on hypothalamic protein levels after 1 h (B). Effect of ICV Sp-cAMPS (90 ng/rats) on cumulative food intake in rats fed a HFD after 2, 4, 6, 8 and 24 h (C). Effect of the injection of lentiviral particles encoding a green fluorescent protein (GFP) or the glucagon receptor in the ARC (D), glucagon protein levels in the ARC (E) and cumulative food intake after overnight fasting (12 h) after the injection of lentiviruses encoding shRNA against glucagon receptor into the ARC of rats fed a chow diet (F). β-actin was used to normalize protein levels. Dividing lines indicate spliced bands from the same gel. Values are mean ± SEM of 7–8 animals per group. *P < 0.05; **P < 0.01; ***P < 0.001.