Figure S3.

Metabolomics analysis of hepatocytes expressing FXRα1 or FXRα2. (A) Amino acid profile determined by mass spectrometry in hepatocytes expressing FXRα1, FXRα2, or GFP alone. The data presented is the same as in Figure 3B but plotted are the relative levels of each individual amino acid analyzed. (B) Analysis of metabolomics data by Ingenuity Pathway Analysis (IPA). Represented are canonical significantly associated with the expression of one or more FXR isoforms. (C) Predicted effects on hepatic metabolism for each FXR isoform. Z-score values indicate induction (orange) or repression (blue). (D) Levels of l-carnitine and acylcarnitines in hepatocytes expressing FXRα1, FXRα2, or GFP alone. Bars represent mean values (n = 1).