Figure S4.

Changing FXRα2/α1 ratios promotes transcriptional reprogramming. (A) Target gene expression analysis by qRT-PCR in hepatocytes expressing GFP or FXRα2 and treated with vehicle (DMSO) or 250 μM cholic acid (CA) for 24h. Bars depict mean values and error bars represent SEM (n = 5). *p < 0.05 compared to vehicle-treated or between the indicated groups. (B) Gene expression analysis by qRT-PCR in hepatocytes co-transduced with FXRα1 and FXRα2 in different proportions (+++, 100%; ++, 70%; +, 30%; −, 0%). Graphs represent expression levels relative to hepatocytes transduced with FXRα1 alone. Bars depict mean values and error bars represent SEM (n = 5). *p < 0.05 compared to FXRα1 alone or between the indicated groups.