Figure 3.

FXRα2 promotes FA oxidation and β-OHB production. (A–F) Hepatocytes were transduced with GFP alone (control) or together with FXRα1 or FXRα2. (A–B) Metabolomics analysis. Metabolite levels (A) and amino acid profile (B) determined by mass spectrometry. Graphs show metabolite levels in cells expressing FXRα1 or FXRα2 relative to control. Amino acids are grouped according to their potential for gluconeogenesis (blue), ketogenesis (red), or both (green) (n = 1). (C–E) Metabolic fluxes. FA oxidation (C), β-OHB release (D), and glucose output (E). (F) Gene expression analysis by qRT-PCR of key mediators of hepatic FA oxidation, mitochondrial uncoupling and ketogenesis. Bars depict mean values and error bars represent SEM (n ≥ 3). *p < 0.05 compared to control (GFP) or between the indicated groups.